Phenotypic Characterization of LEA Rat: A New Rat Model of Nonobese Type 2 Diabetes

Figure 4

Histopathological appearance of pancreatic islets of male LEA rats. At 2 months of age, H&E staining (a), staining with anti-insulin antibody (b), and staining with anti-ED1 antibody (c). At 12 months of age, H&E staining (d and f) and staining with anti-insulin antibody (e and g). Sections (b), (e), and (g) correspond to sections of (a), (b), and (f). Scale bar = 100 μm. (h) Changes in the proportions of 𝛽-cells in the pancreas with age. The areas of the 𝛽-cells and pancreas were calculated by computer-aided imaging. Open and closed bars indicate male LEA and Wistar rats, respectively, at 2 months and 6 months of age. Each value is expressed as the mean ± SD. *𝑃<0.05. **𝑃<0.01.

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