Adipocytes from New Zealand Obese Mice Exhibit Aberrant Proinflammatory Reactivity to the Stress Signal Heat Shock Protein 60
Figure 1
Hsp60 binding to C57BL/6J, NOD, and NZO mouse-derived (pre-)adipocytes. Preadipocytes (a, c, e) or adipocytes (b, d, f) of the three mouse strains were incubated with 100 nM DyLight649-labeled Hsp60 (Hsp60*) in the absence or presence of 1 μM unlabeled Hsp60 or OVA. Binding of Hsp60* was quantified by determining the mean fluorescence intensities (geo mean) of the cell populations using FACS analysis. The data show representative results of Hsp60* binding and inhibition studies (a–f) and of Hsp60* binding to C57BL/6J (light grey bars), NOD (dark grey bars), and NZO mouse-derived cells (black bars) as means + SEM of three independent experiments (g). The specificity of Hsp60* binding was proven by analyses of the effects of unlabelled Hsp60 or OVA (h). The data represent residual Hsp60* binding to C57BL/6J (light grey bars), NOD (dark grey bars), and NZO mouse-derived cells (black bars) as percent of Hsp60* binding (set as 100%, white bar) in the absence of Hsp60 or OVA. The data show means + SEM of three independent experiments. *, **, *** compared to other mouse strains (g) or compared to Hsp60* binding in the absence of Hsp60 or OVA (h).
