Adipocytes from New Zealand Obese Mice Exhibit Aberrant Proinflammatory Reactivity to the Stress Signal Heat Shock Protein 60

Figure 4

Hsp60-induced release of inflammatory mediators by (pre-)adipocytes from C57BL/6J, NOD, and NZO mice. (Pre-)adipocytes from C57BL/6J (light grey bars) NOD (dark grey bars), and NZO mice (black bars), were incubated in the absence (a, b) or presence (c–h) of increasing concentrations of Hsp60 (1–20 μg/mL) and the accumulation of KC, IL-6, and MCP-1 in the culture supernatants was analyzed by multiplex-beads-assay after 24 h. The results show concentrations of accumulated cytokines (a, b) or of cytokine release relative to the cytokine levels detected in the supernatants of unstimulated cells (c–h). The data represent means + SEM of three independent experiments, each performed in triplicates. *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001 compared to other mouse strains.