Adipocytes from New Zealand Obese Mice Exhibit Aberrant Proinflammatory Reactivity to the Stress Signal Heat Shock Protein 60

Figure 5

Hsp60 activates proinflammatory signaling in C57BL/6J, NOD, and NZO mouse-derived (pre-)adipocytes. Preadipocytes (a) and mature adipocytes (b) from C57BL/6J (light grey bars), NOD (dark grey bars), and NZO mice (black bars) were exposed to Hsp60 (10 μg/mL) and after 15 min the activation (phosphorylation) of the MAP-kinases ERK1/2, p38, JNK(p46), and the transcription-factor NFκB was analyzed by immunoblotting. The results show the activation levels of the signaling molecules relative to their activation in cells incubated in the absence of Hsp60. Data represent means + SEM from three independent experiments. *𝑃<0.05, **𝑃<0.01; ***𝑃<0.001 compared to medium control set as 1.

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