Ang (1–7) Protects Islet Endothelial Cells from Palmitate-Induced Apoptosis by AKT, eNOS, p38 MAPK, and JNK Pathways
Figure 1
The effects of palmitate on the RAS in cultured MS-1 cells. Cells were incubated for 24 or 48 h with 0.25% BSA (CON) or palmitate (PA, 0.25 mmol/L). ACE2 and Mas receptor mRNA levels were measured (a). Cells were incubated for 24 h with palmitate (PA, 0.25 mmol/L), alone or in combination with Ang (1–7) (10−6 mol/L). Representative western blots showing the protein expression levels of ACE2, Mas, ACE, and AT1R (b). RT-PCR showing the mRNA levels of ACE2 and Mas receptor (c). Values are mean ± SEM, for each group. # versus CON of 24 h; * versus CON of 48 h.
