Research Article
A Modified Method of Insulin Producing Cells’ Generation from Bone Marrow-Derived Mesenchymal Stem Cells
Figure 1
Detection of C-peptide expression by flow cytometry. ((a), (b)) The dot plots show representative data for one sample, illustrating the analysis of C-peptide of proinsulin in MSCs differentiating into insulin producing cells (IPSs). Indirect, intracellular flow cytometry analysis of fixed and permeabilized insulin producing cells (IPSs) stained with mouse anti-human C-peptide of proinsulin primary antibody. Samples treated with primary antibody were next exposed to (a) goat anti-mouse monoclonal IgG1 FITC or (b) FITC-conjugated goat anti-mouse IgG F()2. (c) C-peptide expression in differentiated IPCs from next culture plotted as a histogram. Grey histogram represents an isotype control. Open histograms represent the expression of C-peptide staining with goat anti-mouse monoclonal IgG1 FITC (black solid line) or with FITC-conjugated goat anti-mouse IgG F()2 (dotted line). The numbers in histograms are the percentage of C-peptide positive cells from samples stained with secondary IgG1 or IgG F()2 (between brackets). The -axis corresponds to logarithmic fluorescence intensity and the -axis to relative cell number.
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