Research Article

A Modified Method of Insulin Producing Cells’ Generation from Bone Marrow-Derived Mesenchymal Stem Cells

Figure 3

Morphological changes of mesenchymal stem cells during 18 days of differentiation. When observed under an inversed microscope, undifferentiated MSCs looked like long spindle-shaped fibroblastic cells and cultures showed a confluency (1st day). Some cells did not change their morphology during 18 days of differentiation, but many of them migrated (8th day). Cells aggregated in clusters and formed islet-like structures (10th day). Cells created colonies from 10th day and continued to 18th day (×40 magnification).
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