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Journal of Diabetes Research
Volume 2014, Article ID 703589, 9 pages
Research Article

Loss of Insulin Receptor in Osteoprogenitor Cells Impairs Structural Strength of Bone

1Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA
2Arkansas Children’s Hospital, 1 Children’s Way, Slot 512-6, Little Rock, AR 72202, USA
3Arkansas Children’s Hospital Research Institute, Little Rock, AR, USA
4Division of Diabetes, Endocrinology & Metabolism, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN, USA
5Joslin Diabetes Center and Harvard Medical School, Boston, MA, USA
6VA Tennessee Valley Health Care System, Vanderbilt University Medical Center, Nashville, TN, USA
7Department of Orthopaedic Surgery & Rehabilitation, Vanderbilt University Medical Center, Nashville, TN, USA
8Center for Bone Biology, Vanderbilt University Medical Center, Nashville, TN, USA

Received 7 February 2014; Accepted 30 April 2014; Published 18 May 2014

Academic Editor: Md. Shahidul Islam

Copyright © 2014 Kathryn Thrailkill et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

To ascertain if any skeletal differences in the OIRKO mice could be attributed to the Cre transgene alone, selected properties of bone, as determined by µCT and three-point bending, were examined by comparing 8- week old Cre-/- and Cre+/- genotypes. Results are shown in Supplemental Table 1. Those skeletal parameters that were significantly affected in the OIRKO phenotype (Table 1: BV/TV, Tb.Th, SMI, Ma.V, Ct.Th, Imin, Ct.Ar, Slenderness, Ct.TMD, and Stiffness), were not significantly different between Cre-/- and Cre+/- genotypes. Body weight and femur length were also not different between Cre-/- and Cre+/- genotypes. This suggested that the skeletal phenotype of OIRKO mice was accounted for by diminished expression of insulin receptor in osteoblasts. (Abbreviations are defined in Table 1. Significant differences are highlighted in bold font.)

  1. Supplementary Material