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Journal of Diabetes Research
Volume 2014, Article ID 980232, 10 pages
http://dx.doi.org/10.1155/2014/980232
Research Article

Continuous Electrical Current and Zinc Sulphate Administered by Transdermal Iontophoresis Improves Skin Healing in Diabetic Rats Induced by Alloxan: Morphological and Ultrastructural Analysis

1Faculty of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, Brazil
2Graduate Program in General Basis of Surgery, Faculty of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, Brazil
3Municipal Institute of Higher Education, IMES-FAFICA, 55030-902 Catanduva, SP, Brazil
4Department of Surgery and Orthopedics, Faculty of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, Brazil
5Department of Pathology, Faculty of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, Brazil

Received 30 June 2014; Accepted 4 August 2014; Published 28 August 2014

Academic Editor: Hiroshi Okamoto

Copyright © 2014 Lucas Langoni Cassettari et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. W. H. Goodson III and T. K. Hunt, “Wound healing and the diabetic patient,” Surgery Gynecology and Obstetrics, vol. 149, no. 4, pp. 600–608, 1979. View at Google Scholar · View at Scopus
  2. R. G. Spanheimer, G. E. Umpierrez, and V. Stumpf, “Decreased collagen production in diabetic rats,” Diabetes, vol. 37, no. 4, pp. 371–376, 1988. View at Publisher · View at Google Scholar · View at Scopus
  3. A. Tellechea, A. Kafanas, E. C. Leal et al., “Increased skin inflammation and blood vessel density in human and experimental diabetes,” International Journal of Lower Extremity Wounds, vol. 12, no. 1, pp. 4–11, 2013. View at Publisher · View at Google Scholar · View at Scopus
  4. S. Khanna, S. Biswas, Y. Shang et al., “Macrophage dysfunction impairs resolution of inflammation in the wounds of diabetic mice,” PLoS ONE, vol. 5, no. 3, Article ID e9539, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. J. G. Minossi, F. O. Lima, C. A. Caramori et al., “Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats,” Acta Cirurgica Brasileira, vol. 29, no. 2, pp. 118–124, 2014. View at Publisher · View at Google Scholar
  6. D. M. Nathan, M. Bayless, P. Cleary et al., “Diabetes control and complications trial/epidemiology of diabetes interventions and complications study at 30 years: advances and contributions,” Diabetes, vol. 62, no. 12, pp. 3976–3986, 2013. View at Google Scholar
  7. J. F. McMurry Jr., “Wound healing with diabetes mellitus. Better glucose control for better wound healing in diabetes,” Surgical Clinics of North America, vol. 64, no. 4, pp. 769–778, 1984. View at Google Scholar · View at Scopus
  8. S. B. Rabelo, A. B. Villaverde, R. A. Nicolau, M. A. Castillo Salgado, M. D. S. Melo, and M. T. T. Pacheco, “Comparison between wound healing in induced diabetic and nondiabetic rats after low-level laser therapy,” Photomedicine and Laser Surgery, vol. 24, no. 4, pp. 474–479, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. G. Yang, C. Luo, X. Yan, L. Cheng, and Y. Chai, “Extracorporeal shock wave treatment improves incisional wound healing in diabetic rats,” The Tohoku Journal of Experimental Medicine, vol. 225, no. 4, pp. 285–292, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. D. G. Greenhalgh, K. H. Sprugel, M. J. Murray, and R. Ross, “PDGF and FGF stimulate wound healing in the genetically diabetic mouse,” The American Journal of Pathology, vol. 136, no. 6, pp. 1235–1246, 1990. View at Google Scholar · View at Scopus
  11. P. Inpanya, A. Faikrua, A. Ounaroon, A. Sittichokechaiwut, and J. Viyoch, “Effects of the blended fibroin/aloe gel film on wound healing in streptozotocin-induced diabetic rats,” Biomedical Materials, vol. 7, no. 3, Article ID 035008, 13 pages, 2012. View at Publisher · View at Google Scholar
  12. P. Velander, C. Theopold, O. Bleiziffer et al., “Cell suspensions of autologous keratinocytes or autologous fibroblasts accelerate the healing full thickness skin wounds in a diabetic wound healing,” Journal of Surgical Research, vol. 157, no. 1, pp. 14–20, 2009. View at Google Scholar
  13. L. C. Kloth, “Electrical stimulation for wound healing: a review of evidence from in vitro studies, animal experiments, and clinical trials,” International Journal of Lower Extremity Wounds, vol. 4, no. 1, pp. 23–44, 2005. View at Publisher · View at Google Scholar · View at Scopus
  14. N. Dixit, V. Bali, S. Baboota, A. Ahuja, and J. Ali, “Iontophoresis—an approach for controlled drug delivery: a review,” Current Drug Delivery, vol. 4, no. 1, pp. 1–10, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. A. B. G. Landsdown, “Zinc in the healing wound,” The Lancet, vol. 347, no. 9003, pp. 706–707, 1996. View at Publisher · View at Google Scholar · View at Scopus
  16. S. P. Moraes, F. R. B. Chaves, S. Banci, P. A. Rover, F. Georgetti, and J. A. Reis-Neto, “The effect of zinc and chromium on wound healing in normal and diabetic rats,” Revista do Colégio Brasileiro de Cirurgiões, vol. 27, no. 5, pp. 394–399, 2000. View at Google Scholar
  17. J. Smith, N. Romansky, J. Vomero, and R. H. Davis, “The effect of electrical stimulation on wound healing in diabetic mice,” Journal of the American Podiatry Association, vol. 74, no. 2, pp. 71–75, 1984. View at Publisher · View at Google Scholar · View at Scopus
  18. P. Y. Lee, S. Chesnoy, and L. Huang, “Electroporatic delivery of TGF-beta1 gene works synergistically with electric therapy to enhance diabetic wound healing in db/db mice,” Journal of Investigative Dermatology, vol. 123, no. 4, pp. 791–798, 2004. View at Publisher · View at Google Scholar
  19. H. A. Thawer and P. E. Houghton, “Effects of stimulation the histological properties of wounds in,” Wound Repair Regen, vol. 9, no. 2, pp. 107–115, 2001. View at Google Scholar
  20. L. L. Cassettari, Efeitos da corrente elétrica contínua isolada e da iontoforese transdermal, associada ao zinco, sobre a resistência mecânica e o conteúdo de hidroxiprolina de cicatrizes realizadas na pele de ratos diabéticos aloxânicos [Mestrado em Agressão, Reparação, Regeneração e Transplantes de Tecidos e Órgãos], Faculdade de Medicina da UNESP, 2010.
  21. M. C. Komesu, M. B. Tanga, K. R. Buttros, and C. Nakao, “Effects of acute diabetes on rat cutaneous wound healing,” Pathophysiology, vol. 11, no. 2, pp. 63–67, 2004. View at Publisher · View at Google Scholar · View at Scopus
  22. A. Barbul and M. C. Regan, “Immune involvement in wound healing,” Otolaryngologic Clinics of North America, vol. 28, no. 5, pp. 955–968, 1995. View at Google Scholar · View at Scopus
  23. S. E. Mutsaers, J. E. Bishop, G. McGrouther, and G. J. Laurent, “Mechanisms of tissue repair: from wound healing to fibrosis,” International Journal of Biochemistry and Cell Biology, vol. 29, no. 1, pp. 5–17, 1997. View at Publisher · View at Google Scholar · View at Scopus
  24. T. A. Mustoe, G. F. Pierce, A. Thomason, P. Gramates, M. B. Sporn, and T. F. Deuel, “Accelerated healing of incisional wounds in rats induced by transforming growth factor-β,” Science, vol. 237, no. 4820, pp. 1333–1336, 1987. View at Publisher · View at Google Scholar · View at Scopus
  25. V. Falanga, “Growth factors and wound healing,” Dermatologic Clinics, vol. 11, no. 4, pp. 667–675, 1993. View at Google Scholar · View at Scopus
  26. M. Delamaire, D. Maugendre, M. Moreno, M. C. Le Goff, H. Allannic, and B. Genetet, “Impaired leucocyte functions in diabetic patients,” Diabetic Medicine, vol. 14, no. 1, pp. 29–34, 1997. View at Google Scholar
  27. R. Blakytny and E. Jude, “The molecular biology of chronic wounds and delayed healing in diabetes,” Diabetic Medicine, vol. 23, no. 6, pp. 594–608, 2006. View at Publisher · View at Google Scholar · View at Scopus
  28. R. A. F. Clark, “Cutaneous tissue repair: basic biologic considerations. I,” Journal of the American Academy of Dermatology, vol. 13, no. 5, part 1, pp. 701–725, 1985. View at Publisher · View at Google Scholar · View at Scopus
  29. E. V. P. Ortolan, C. T. Spadella, C. Caramori, J. L. M. Machado, E. A. Gregorio, and K. Rabello, “Microscopic, morphometric and ultrastructural analysis of anastomotic healing in the intestine of normal and diabetic rats,” Experimental and Clinical Endocrinology and Diabetes, vol. 116, no. 4, pp. 198–202, 2008. View at Publisher · View at Google Scholar · View at Scopus
  30. N. N. Rizk, “Scanning electron microscopy of the structural reconstruction of the abdominal wall after experimental paramedian incision,” Journal of Surgical Research, vol. 35, no. 4, pp. 354–364, 1983. View at Publisher · View at Google Scholar · View at Scopus
  31. N. A. Parizotto, Acão do laser Hélio-Neônio sobre o processo de raparo tecidual: Um estudo do colágeno por microscopia eletrônica de varredura, microscopia de força atômica e espectroscopia por infravermelho [Doutorado em Engenharia Elétrica], Universidade de Campinas, 1998.
  32. W. K. Beheregaray, G. C. Gianotti, J. S. Leal, T. Garcez, and E. A. Contesini, “Electrical stimulation in experimental wound healing in rabbits,” Ciência Rural, vol. 44, no. 5, pp. 878–883, 2014. View at Google Scholar
  33. B. Priya, T. Rashmi, and M. Bozena, “Transdermal iontophoresis,” Expert Opinion on Drug Delivery, vol. 3, no. 1, pp. 127–138, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. H. Onodera, D. Ikeuchi, S. Nagayama, and M. Imamura, “Weakness of anastomotic site in diabetic rats is caused by changes in the integrity of newly formed collagen,” Digestive Surgery, vol. 21, no. 2, pp. 146–151, 2004. View at Publisher · View at Google Scholar · View at Scopus
  35. M. S. Agren, T. A. Soderberg, C.-O. Reuterving, G. Hallmans, and I. Tengrup, “Effect of topical zinc oxide on bacterial growth and inflammation in full-thickness skin wounds in normal and diabetic rats,” Acta Chirurgica—European Journal of Surgery, vol. 157, no. 2, pp. 97–101, 1991. View at Google Scholar · View at Scopus
  36. M. S. Agren, M. Chvapil, and L. Franzén, “Enhancement of re-epithelialization with topical zinc oxide in porcine partial-thickness wounds,” Journal of Surgical Research, vol. 50, no. 2, pp. 101–105, 1991. View at Publisher · View at Google Scholar · View at Scopus
  37. M. S. Agren, “Studies on zinc in wound healing,” Acta Dermato-Venereologica Supplement, vol. 69, no. 154, pp. 1–36, 1990. View at Google Scholar · View at Scopus
  38. J. N. Norman, R. Assadulah, and G. Smith, “Effect of supplements of zinc salts on the healing of granulating wounds in the rat and guinea pig,” Journal of Nutrition, vol. 105, no. 7, pp. 815–821, 1975. View at Google Scholar · View at Scopus
  39. M. W. Cornwall, “Zinc iontophoresis to treat ischemic skin ulcers,” Physical Therapy, vol. 61, no. 3, pp. 359–360, 1981. View at Google Scholar · View at Scopus
  40. F. Esen, S. Güleç, and H. Esen, “Exogenous zinc improves blood fluidity but has no effect on the mechanisms of vascular response to acetylcholine iontophoresis in humans,” Biological Trace Element Research, vol. 113, no. 2, pp. 139–153, 2006. View at Publisher · View at Google Scholar · View at Scopus
  41. L. L. Cassettari, P. C. R. Dias, A. N. Lucchesi, M. F. de Arruda, and C. T. Spadella, “Zinc sulphate administered by transdermal iontophoresis improves breaking strength of surgical wounds in skin of alloxan-induced diabetic rats,” Acta Cirurgica Brasileira, vol. 28, no. 8, pp. 601–606, 2013. View at Publisher · View at Google Scholar · View at Scopus