Inhibition of Ceramide De Novo Synthesis Ameliorates Diet Induced Skeletal Muscles Insulin Resistance

Table 2

Effect of myriocin treatment (for 7 days) and high fat diet feeding (for 5 weeks) on the neutral (nSMase) and acidic (aSMase) sphingomyelinases activities in rat skeletal muscle [ (per group) = 8].

	nSMase (nmol/h/mg protein)				aSMase (nmol/h/mg protein)
	Control		HFD		Control		HFD
	M−	M+	M−	M+	M−	M+	M−	M+
Soleus	4.6 ± 1.6	3.9 ± 0.8	4.7 ± 1.5	2.9 ± 0.9	13.4 ± 3.4	14.6 ± 2.7	12.3 ± 2.1	14.1 ± 2.6
Red gastrocnemius	4.6 ± 1.2	5.2 ± 0.7	4.4 ± 0.8	2.6 ± 0.5	6.7 ± 0.8	7.5 ± 1.4	7.2 ± 1.0	7.4 ± 1.3
White gastrocnemius	4.8 ± 0.6	5.7 ± 1.4	4.2 ± 1.0	3.2 ± 1.2	4.5 ± 1.3	6.0 ± 1.6	5.4 ± 0.7	5.0 ± 1.6

HFD: group fed with high fat diet. M+: subgroup additionally treated with myriocin. M−: subgroup untreated with myriocin. Results are expressed as means ± SD.
compared with control group.
^† compared with HFD group.