Inhibition of Ceramide De Novo Synthesis Ameliorates Diet Induced Skeletal Muscles Insulin Resistance
Table 3
Effect of myriocin treatment (for 7 days) and high fat diet feeding (for 5 weeks) on the neutral (nCer-ase), alkaline (alkCer-ase), and acidic (aCer-ase) ceramidases activities in rat skeletal muscle [ (per group) = 8].
nCer-ase (nmol/h/mg protein)
alkCer-ase (nmol/h/mg protein)
aCer-ase (nmol/h/mg protein)
Control
HFD
Control
HFD
Control
HFD
M−
M+
M−
M+
M−
M+
M−
M+
M−
M+
M−
M+
Soleus
0.43 ± 0.2
0.16 ± 0.1
0.75 ± 0.2
0.15 ± 0.1
0.55 ± 0.2
0.21 ± 0.1
0.87 ± 0.3
0.28 ± 0.1
0.58 ± 0.3
0.45 ± 0.2
0.49 ± 0.2
0.51 ± 0.3
Red gastrocnemius
0.38 ± 0.2
0.24 ± 0.1
0.66 ± 0.1
0.21 ± 0.1
0.48 ± 0.1
0.16 ± 0.1
0.78 ± 0.2
0.15 ± 0.1
0.41 ± 0.2
0.48 ± 0.3
0.45 ± 0.1
0.52 ± 0.2
White gastrocnemius
0.17 ± 0.1
0.19 ± 0.1
0.16 ± 0.1
0.20 ± 0.1
0.24 ± 0.1
0.28 ± 0.1
0.18 ± 0.1
0.21 ± 0.1
0.21 ± 0.1
0.17 ± 0.1
0.20 ± 0.1
0.16 ± 0.1
HFD: group fed with high fat diet. M+: subgroup additionally treated with myriocin. M−: subgroup untreated with myriocin. Results are expressed as means ± SD. compared with control group. † compared with HFD group.
