Research Article

Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human Pancreatic β Cells

Figure 2

Effects of Dx and IL-6 on the promoter activity of (a) REG Iα, (b) REG Iβ, (c) REG III, (d) HIP/PAP, and (e) REG IV in rat RINm5F cells. RINm5F cells were transfected with the human REG family gene reporter plasmids and treated without (no addition) or with Dx (100 nM), or IL-6 (20 ng/mL), or IL-6 + Dx. The promoter activity was normalized for variations in transfection efficiency using β-galactosidase activity as an internal standard. Fold increase is calculated by dividing the promoter activity of stimulated cells by that of unstimulated cells (No addition). Data are expressed as means ± SE for each group (). ; .
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