Therapeutic Benefit of Extended Thymosin β4 Treatment Is Independent of Blood Glucose Level in Mice with Diabetic Peripheral Neuropathy
Effect of Tβ4 on DRG neurons neurite outgrowth in vitro. Panels (a) to (e) show NF-H immunoreactive nondiabetic DRG neuron cultured in normal glucose (N, (a)), high glucose (H, (b)), high glucose with Tβ4 (+, (c)), high glucose with Ang1 (+Ang, 100 ng/mL, (d)), and Tβ4 with antibody against Tie2 (+Tie, 5 μg/mL, (e)). Panels (f) to (h) show NF-H immunoreactive diabetic DRG neuron cultured in normal glucose (f), normal glucose with Tβ4 (g), and Tβ4 with antibody against Tie2 (h). Panel (i) shows quantitative data of neurite outgrowth. Bar in μm. versus the nondiabetic DRG neurons (DM) in normal glucose and versus nondiabetic DRG neurons in the high glucose. versus nondiabetic DRG neurons treated with Tβ4 in high glucose, versus diabetic DRG neurons (DB) in normal glucose, and versus diabetic DRG neurons treated with Tβ4. /group.