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Journal of Diabetes Research
Volume 2015, Article ID 176949, 10 pages
Clinical Study

Polymorphisms of GLP-1 Receptor Gene and Response to GLP-1 Analogue in Patients with Poorly Controlled Type 2 Diabetes

1Division of Endocrinology and Metabolism, Department of Internal Medicine, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan
2Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan 333, Taiwan
3Department of Biotechnology, Ming Chuan University, Taoyuan 333, Taiwan
4Genomic Medicine Research Core Laboratory, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan

Received 26 September 2014; Revised 22 December 2014; Accepted 5 January 2015

Academic Editor: Daisuke Koya

Copyright © 2015 Chia-Hung Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The promoter, all 13 exons and intron-exon boundaries of the GLP1R gene (GenBank accession number AL035690) were amplified by polymerase chain reaction (PCR) using specific primer sets in Supplementary Table 1. The Titanium Taq DNA Polymerase (Clontech Laboratories, Inc., Mountain View, CA, USA) was used in PCR. The PCR amplification conditions consisted of initial denaturation at 95°C for 5 minutes followed by 40 cycles at 95°C for 45 seconds, annealing temperature for 45 seconds and 72°C for 45 seconds, with one additional cycle at 72°C for 10 minutes.

  1. Supplementary Material