Angioblast Derived from ES Cells Construct Blood Vessels and Ameliorate Diabetic Polyneuropathy in Mice

Figure 2

Transcript levels of angiogenic and neurotrophic factors in angioblast-like cells induced from ES cells (ES-ABs). Sorted ES-ABs expressed angiogenic and neurotrophic factors: VEGF-A, PDGF-A, FGF2, NGF, brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), Neurotrophin-3 (NT-3), and ciliary neurotrophic factor (CNTF). Each expression level of the factors compared Flk1 positive cells with Flk1 negative cells, PA6 cells, and IMS32 cells. PA6: a cell line of mouse mesenchymal stem cell and IMS32: a cell line of immortalized mouse Schwann cell. Flk1⁺: Flk1 positive cells and Flk1⁻: Flk1 negative cells. versus Flk1⁻ cells, versus PA6 cells, and versus IMS32 cells.