Effects of High Glucose Levels and Glycated Serum on GIP Responsiveness in the Pancreatic Beta Cell Line HIT-T15

Figure 1

Treatment with GS reduces GIPR protein expression in cells cultured under HG. HIT-T15 cells were cultured for 5 days in media containing 5.6 mmol/L (CTR) or 11.1 mmol/L glucose (HG) supplemented with GS. Then cells were lysed and tested for protein expression of GIPR. (a) Representative western blot analysis. (b) Quantification of densitometries of western blot bands. Data were expressed as mean ± SE of fold induction relative to GAPDH (). versus CTR.

(a)

(b)