Alteration of Mevalonate Pathway in Proliferated Vascular Smooth Muscle from Diabetic Mice: Possible Role in High-Glucose-Induced Atherogenic Process
Changes of key enzymes in cultured VSMCs treated with different glucose concentrations for 48 h. (a) Gene expressions from QRT-PCR. The relative expression level of each sample was calibrated by the comparative threshold cycle method, using β-actin as an endogenous control. (b) Protein expressions from western blot analysis. β-actin was used as a loading control. Data expressed as mean ± SEM. and versus normal glucose group (5.6 mmol/L). HMGR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; P-HMGR, phosphor-3-hydroxy-3-methylglutaryl-coenzyme A reductase; FPPS, farnesyl pyrophosphate synthase; SQS, squalene synthase; GGPPS, geranylgeranyl pyrophosphate synthase; FNT-β, farnesyltransferase-β; GGT-1β, geranylgeranyltransferase-1β.