Nuclear Factor of Activated T Cells Is Activated in the Endothelium of Retinal Microvessels in Diabetic Mice

Figure 6

In vivo inhibition of NFAT reduces the expression of OPN and ICAM-1 in retinal vessels. mRNA expression of (a) OPN and (b) ICAM-1 in isolated retinal vessels from BALB/c mice treated with STZ (60 mg/kg) or vehicle (citrate buffer). Mice received for 4 weeks daily i.p. injections of A-285222 (0.29 mg/kg for 2 weeks followed by 0.15 mg/kg until termination) or vehicle (saline). Expression levels were determined by quantitative RT-PCR and HPRT was used as endogenous control. -8 mice/group. Two-way ANOVA revealed a significant effect of A-285222 on OPN and ICAM-1 expression; .