Maternal Hyperglycemia Directly and Rapidly Induces Cardiac Septal Overgrowth in Fetal Rats

Table 1

List of RT-PCR primers.

Gene	NCBI GeneID	Forward	Reverse	Amplicon size (bp)
Glut1	24778	CTTTGGCAGGCGGAACTC	TTGCCCAGTTTGGAGAAACC	84
Actb	81822	TTCCTTCCTGGGTATGGAATCC	GGATGTCAACGTCACACTTCATG	77
Hk2	25059	TGGGCTGGACAACCTCAAAG	CCTTGGCAAAGTGAGGATGAAG	74
Pdk1	116551	GCGAGACGGCTTTGTGATTTG	CCTGGTGATTTCGCATTTAGTTC	77
P53	24842	CACTCCAGCTACCCGAAGAC	GCCAGGAACCAGTTTGCATAG	259
Ldha	24533	AATGAAGGACTTGGCTGATGAG	GCCATGCTGAAGATCCATCATC	85
Tigar	502894	ACGCCTTCTCCAGTGATCTC	CGTACATCCTTTCCCGAAGTC	120
Gpt2	307759	CCCACAGGCCAGGTACAAAG	AAAGCTTCTCTTCCCAGGCAAAG	70
Pkm2	25630	TGACACCTTCCTGGAACACATG	GGGAAGCAGGGCCAATGG	98

Gene abbreviations: Glut1, solute carrier family 2 (facilitated glucose transporter) member 1; Actb, beta actin; Hk2, hexokinase 2; Pdk1, pyruvate dehydrogenase kinase isozyme 1; P53, tumor protein p53; Ldha, lactate dehydrogenase A; Tigar, fructose-2,6-bisphosphatase TIGAR; Gpt2, glutamic pyruvate transaminase (alanine aminotransferase) 2; Pkm2, pyruvate kinase muscle.