Research Article

Sex-Differences in Renal Expression of Selected Transporters and Transcription Factors in Lean and Obese Zucker Spontaneously Hypertensive Fatty Rats

Figure 4

IC50 determination for the inhibition of OAT2-mediated cGMP uptake by furosemide and bumetanide. In HEK293 cells stably transfected with OAT2 or empty vector, intracellular cGMP accumulation was determined after coincubation with 10 μM cGMP (0.1 μM cGMP + 9.9 μM unlabeled cGMP) and 1–1000 μM furosemide (a) or 10–1000 μM bumetanide (b), respectively, for 5 min at 37°C. The furosemide and bumetanide concentrations causing half-maximal inhibitory effect (IC50) on cGMP accumulation in OAT2 expressing cells were calculated. Data are presented as mean ± SEM. ; .
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