NADPH Oxidase-Induced NALP3 Inflammasome Activation Is Driven by Thioredoxin-Interacting Protein Which Contributes to Podocyte Injury in Hyperglycemia

Figure 6

Inhibition of TXNIP abolishes the HG-triggered upregulation of . (a) Western blot analysis showing the expression of in HG-exposed podocytes without or with TXNIP shRNA transfection. (b) Summarized data showing the band intensities measured from (). (c) Western blot analysis showing the expression of TXNIP in HG-stimulated podocytes without or with shRNA transfection. (d) Summarized data showing the band intensities measured from TXNIP (). (e) Protein expression of TXNIP in HG-stimulated podocytes without or with pretreatment of APO or DPI. (f) Summarized data showing the band intensities of TXNIP ( = 4–6). Ctrl: control; HG: high glucose; Veh: vehicle; Scram: scrambled shRNA; shTXNIP: TXNIP shRNA; shgp91: shRNA; APO: apocynin; DPI: diphenyleneiodonium. compared with Ctrl; compared with HG group treated with vehicle or transfected with scramble shRNA.

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