Research Article
Dendrobium chrysotoxum Lindl. Alleviates Diabetic Retinopathy by Preventing Retinal Inflammation and Tight Junction Protein Decrease
Table 1
List of primers for real-time PCR.
| | Target | Gene ID | Primer | Sequence |
| | ICAM1 | 25464 | FP | 5′-TGCAGGTGAACTGCTCTTCCTCTT-3′ | | RP | 5′-AGCTTCCAGTTGTGTCCACTCGAT-3′ |
| | Tnf | 24835 | FP |
5′-CCACCACGCTCTTCTGTCTACTG-3′ | | RP | 5′-GGGCTACGGGCTTGTCACTC-3′ |
| | Il6 | 24498 | FP | 5′-TCCTACCCCAACTTCCAATGCTC-3′ | | RP | 5′-TTGGATGGTTCTTGGTCCAATGCTC-3′ |
| | Il1b | 24494 | FP | 5′-TGTCACTCATTGTGGCTGTGGAGA-3′ | | RP | 5′-TGGGAACATCACACACTAGCAGGT-3′ |
| | Ocln | 83497 | FP | 5′-TGGGACAGAGCCTATGGAACG-3′ | | RP | 5′-ATCACCAAGGAAGCGATGAAGC-3′ |
| | Tjp1 | 292994 | FP | 5′-GATGAGCGGGCTACCTTATTGA-3′ | | RP | 5′-AGCGAACTGAATGGTCTGATGC-3′ |
| | Cldn1 | 65129 | FP | 5′-TGCCCTACTTTCCTGCTCCTGT-3′ | | RP | 5′-AGTAGAAGGTGTTGGCTTGGGATAA-3′ |
| | Cldn5 | 65131 | FP | 5′-CAGCGTTGGAAATTCTGGGTCT-3′ | | RP | 5′-CGTCTGCGCCGTCACGATAT-3′ |
| | Actb | 81822 | FP | 5′-CGCTCGTCGTCGACAACGG-3′ | | RP | 5′-TGTGGTGCCAAATCTTCTCC-3′ |
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FP: forward primer; RP: reverse primer.
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