Diabetic Nephropathy Induced by Increased Ace Gene Dosage Is Associated with High Renal Levels of Angiotensin (1–7) and Bradykinin
Table 1
Main physiologic and biochemical parameters of control and diabetic groups at first week and 12 weeks after induction of diabetes.
Parameter/group
First week of experimental protocol
Twelfth week of experimental protocol
1 copy
3 copies
1 copy
3 copies
Control
Diabetic
Control
Diabetic
Control
Diabetic
Control
Diabetic
Body weight (g)
24.6 ± 0.1
26.3 ± 0.5
25.1 ± 0.8
26.8 ± 0.7
28.5 ± 0.6
23.7 ± 1.
30.0 ± 1.1
25.1 ± 0.
Plasma glucose (mg/dL)
113 ± 5
348 ± 1
106 ± 4
355 ± 3
132 ± 7.30
499 ± 2
133 ± 3
459 ± 2
Food intake (g/24 h)
2.5 ± 0.2
4.5 ± 0.
3.50 ± 0.4
4.2 ± 0.
2.8 ± 0.4
4.7 ± 0.
2.9 ± 0.2
6.1 ± 0.
Water intake (mL/24 h)
4.6 ± 0.5
17.7 ±
5.4 ± 0.3
14.0 ± 2.
4.0 ± 0.6
17.6 ± 3.
5.9 ± 0.8
25.7 ± 2.
Urine volume (mL/24 h)
0.6 ± 0.1
10.8 ± 1.
1.3 ± 0.1
11.8 ± 2.
0.7 ± 0.1
13.4 ± 2.
1.1 ± 0.2
20.6 ± 2.
Relative kidney weight (g/g × 1000)
—
—
—
—
5.8 ± 0.2
8.6 ± 0.
5.6 ± 0.2
7.2 ± 0.
Albuminuria (mg/24 h)
1.1 ± 0.2
1.7 ± 0.9
1.7 ± 0.2
2.4 ± 0.1
1.4 ± 0.2
2.1 ± 0.8
1.7 ± 0.3
4.9 ± 1.
Urinary urea (mg/24 h)
Urinary creatinine (mg/24 h)
0.40 ± 0.01
0.09 ± 0.0
0.38 ± 0.03
0.04 ± 0.0
0.38 ± 0.06
0.08 ± 0.0
0.39 ± 0.12
0.06 ± 0.0
Values represent mean ± S.E.M., = 6–11. Two-way ANOVA or two-way ANOVA for repeated measures (whether necessary) showed : diabetic versus control, : 3 copies diabetic group versus 1 copy diabetic group, : versus same diabetic group at first week.
