|ND = not detectable. This table is adapted from our previous studies [27, 65, 91].|
The chemical composition of control diet was as follows (g/kg dry diet): starch, 587; casein, 200; cellulose, 50; sucrose, 50; mineral mix, 40; vitamin mix, 20; DL-methionine, 3; vegetable oil-Isio-4 (Lesieur, Neuilly-sur-Seine, France), 50. Total oil represented 5% of the diet. In the omega-3 PUFA diet, half of the vegetable oil-Isio-4 was replaced by EPAX-7010 (the omega-3 PUFA oil). The vegetable Isio-4 oil contained the following: 47.2 mg/g 18:2 (n-6); 1.7 mg/g total (n-3); and 40.2 mg/g monounsaturated fatty acids (largely 18:1). EPAX-7010 oil, in the form of ethyl ester, contained approximately 85% (n-3) PUFA, that is, EPA, 70%, DHA, 12%, and -tocopherol, 2.1 to 3.2%. It means that EPAX oil represented 2.5% of the diet. Since the omega-3 PUFA consisted of 85% of the 2.5% EPAX oil, the total n-3 PUFA represented only 2.1% of the total diet. After diets’ preparation, the lipids from diets were extracted according to the method described in Yessoufou et al., 2006 , and then transmethylated by BF3/methanol after saponification, and fatty acids were analysed by gas liquid chromatography.