Figure 2: Abnormal sugar metabolism is observed in the imp knockdown larva. (a) imp transcript was analyzed by q-PCR in larvae of the indicated genotypes. It was confirmed that the CNS specific knockdown of imp led to reduction of imp to about one-fifth of that of the control strain. (b) Normalized trehalose in whole larvae homogenized preparation was compared in order to examine the overall carbohydrate metabolism with protein concentration as an internal reference. The imp mutant shows a delay in trehalose usage in the fasting condition (7-hour fast, ). (c) Hemolymph trehalose concentration is increased in the imp mutant for both fed and starved condition. The difference is more prominent after 15-hour fasting (). . (d) In contrast, hemolymph protein concentration was unchanged for both fed and starved condition.