In Vitro and In Vivo Effects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue

Figure 1

The results of mice adipose-derived mesenchymal stem cells immunophenotyping represented in images. Immunostaining analysis was performed on three ASCs cultures, after second passage. The obtained populations of cells were positive for markers characteristic for mesenchymal cells, that is, CD29, CD44, CD73, and CD105, and were negative for hematopoietic marker CD45. Particular markers were stained with specific primary antibody and secondary antibody conjugated with atto-488 (positive reactions shown in green), and nuclei stained with DAPI are shown in white. Results of negative staining are included in the Figure. Magnification used is 100x. Scale bar = 250 μm.