In Vitro and In Vivo Effects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue

Figure 11

Results of immunohistochemical staining for CD44, CD105, OPN, Ki67, and caspase-3 (CASP-3). In order to recognize the nuclei location, the images of specific staining were arranged with images presenting results of DAPI staining (blue-tone images). The weaker CD44⁺, CD105⁺, OPN⁺, and Ki67⁺ reactions were noted in subcutaneous fat tissues collected from experimental group. Stronger positive reaction for caspase-3 was seen in fat biopsies derived from animals treated with metformin than in control group. Immunostaining reactions were prepared in duplicate. Magnifications are 50x and 100x; scale bar is 400 μm.