Journal of Diabetes Research / 2015 / Article / Fig 4

Research Article

The PI3K/Akt Signaling Pathway Mediates the High Glucose-Induced Expression of Extracellular Matrix Molecules in Human Retinal Pigment Epithelial Cells

Figure 4

(a) Activation of Akt by high glucose in RPE cells. PRE cells were stimulated with high glucose (25 mM) for the indicated periods of time, and cell lysates were immunoblotted with anti-p-Akt and anti-Akt antibodies. versus NG. (b) RPE cells were pretreatment with 20 μM LY294002 for 30 min and then incubated with high glucose for 30 min for assay of Akt phosphorylation. Levels of phosphorylated Akt were determined with western blot analysis. The data represent the deviation (SD) of three independent experiments. versus NG or .