MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue

Figure 2

miR-223 regulates GLUT4 expression in human differentiated adipocytes. (a), (c), and (d) Depicted are the miR-223, GLUT4 protein, and GLUT4 gene expression in human differentiated adipocytes after being transfected with miR-223 overexpression plasmid. (b) Depicted is 6-NBDG uptake stimulated by insulin in human differentiated adipocytes after being transfected with miR-223 overexpression plasmid. (e) Depicted is a GLUT4 3′UTR predicted binding site for miR-223. (f) Depicted is the GLUT4 3′UTR luciferase reporter which was repressed by overexpression of miR-223. comparing to empty plasmid. (In (a), (c), (d), and (f), the comparisons of significance were carried out by unpaired Student’s -test. In (b), the comparison of significance was carried out by ANOVA followed by a posttest by using the Fisher (among groups) and Dunnett (compared to control group) test.)

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