Charge-Based Inhibitors of Amylin Fibrillization and Toxicity

Figure 10

Effects of charge-based inhibitors on amylin cytotoxicity. (a) Cytotoxicity of WT-amylin and analogs towards a MIN6 mouse insulinoma cell model of -pancreatic cells [18]. Gray, cells-only, defined as 100% cell viability and 1% DMSO vector used for all experiments except the first; brown, positive melittin control; orange, WT-amylin at different concentrations; blue, green, red-cells challenged with 80 M Arg-1, Arg-2, Mem-T respectively; light blue, green, red-cells challenged with 80 M WT-amylin and the indicated molar ratios of the respective inhibitors. (b) Viability of MIN6 cells challenged with 80 M WT-amylin as a function of Arg-1 concentration. The cell viability data were fit to an IC₅₀ value of  M Arg-1. All data points are averages from measurements performed in triplicate and the uncertainties are the associated SEMs.

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