Charge-Based Inhibitors of Amylin Fibrillization and Toxicity

Figure 4

Representative reaction profiles showing the effects of peptide inhibitors at the indicated concentrations on the fibrillization of 20 M WT-amylin: (a) Arg-1, (b) Arg-2, and (c) Mem-T. All reactions were done in the presence of 150 mM NaCl. A single-step fibril-formation process was assumed for the analysis of all the kinetic reactions. Although some of the reactions appear to show more complicated fluctuations in the data, these are likely experimental noise (the presence of particulate fibrils leads to nonideality in fluorescence measurements) as they are not observed in replicate measurements.

(a)

(b)

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