JNK1 Deficient Insulin-Producing Cells Are Protected against Interleukin-1β-Induced Apoptosis Associated with Abrogated Myc Expression

Figure 2

IL-1β reduces JNK1 but not JNK2 or JNK3 protein expression and increases P-JNK after 0.5 h of exposure in INS-1 cells. (a) INS-1 cells were exposed to 150 pg/mL IL-1β or vehicle for 4 to 24 h. JNK subtypes protein expression, JNK1 (circle), JNK2 (square), and JNK3 (triangle), were measured by immunoblotting and normalized to actin or tubulin. Data are shown as % IL-1β-regulated JNK subtype expression of their respective nonexposed controls. Data are shown as means of independent experiments ± SEM; versus 4 h time point. (b) INS-1 cells were exposed to 150 pg/mL IL-1β or vehicle for 0.5 to 24 h. INS-1 cells exposed for IL-1β for 12 h were lysed at an earlier time point paralleled with the 12 h vehicle ctrl. P-JNK was measured by immunoblotting and normalized to T-JNK. Data are shown as means of independent experiments ± SEM; , , and versus vehicle (0 h). Representative gels are shown.

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