JNK1 Deficient Insulin-Producing Cells Are Protected against Interleukin-1β-Induced Apoptosis Associated with Abrogated Myc Expression

Figure 6

JNK1 knockdown prevents the regulation of total Myc protein by IL-1β. INS-1 cells were exposed to 150 pg/mL IL-1β (closed bars) or vehicle (open bars) for 4 to 24 h. Protein was isolated and (a) total Myc protein levels were analyzed by immunoblotting. Total protein was used as the loading control. Data are shown ± SEM of independent experiments. INS-1 cells stably expressing shRNA directed against JNK1, JNK2, JNK3, or the nonsense or empty vector controls were exposed to 150 pg/mL IL-1β (black bars) or left unexposed (white bars) for 16 h. Protein was isolated, and (b) total Myc protein levels were analyzed by immunoblotting. Actin was used as the loading control. Data are shown ± SEM of independent experiments. and comparing Myc levels in the presence with Myc levels in the absence of IL-1β, unless otherwise indicated.

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