Influence of Aluminium and EGCG on Fibrillation and Aggregation of Human Islet Amyloid Polypeptide

Figure 2

Chelation of Al(III) and EGCG. (a) UV spectra of 50 μM EGCG in the presence of Al(III) at various concentrations from 0 to 150 μM. (b) Dependence of absorbance of 50 μM EGCG at 273 nm (red) and 307 nm (black) on the molar ratios of Al(III) to EGCG from 0 to 3. The values represent mean ± SD (). (c) Fluorescence spectra of 150 μM EGCG as Al(III) concentration increased from 0 to 450 μM. (d) 150 μM EGCG fluorescence intensity at 425 nm on molar ratio of Al(III) to EGCG. The intensity values were normalized to 150 μM EGCG with 450 μM Al(III). The values represent mean ± SD ().

(a)

(b)

(c)

(d)