Journal of Diabetes Research / 2016 / Article / Fig 5

Research Article

ClC-3 Expression and Its Association with Hyperglycemia Induced HT22 Hippocampal Neuronal Cell Apoptosis

Figure 5

Osmotic control experiment: MTT cell viability assay, results of glucose and mannitol (osmotic control) groups obtained at 24 h, 48 h, and 72 h displayed in terms of the percentage of viable cells (% of 0 mM) (a). Western blot analysis of HT22 cell lysates from control, mannitol, and glucose groups, at 24 h, for ClC-3 protein expression (b) and quantification of their respective intensity levels (ratio of ClC-3 band to standard β-actin band) (c) showed a significant increase in the expression level of ClC-3 in high glucose group (). Control: normal osmolarity (25 mM) group, ML: mannitol low osmolarity (30 mM) group, MH: mannitol high osmolarity (50 mM) group, GL: glucose low osmolarity (30 mM) group, and GH: glucose high osmolarity (50 mM) group.