Potentiation of Calcium Influx and Insulin Secretion in Pancreatic Beta Cell by the Specific TREK-1 Blocker Spadin

Figure 5

SPA modulates insulin secretion in mice. (a) IPGTT challenge (2 g/kg glucose i.p.) is performed onto two groups of C57Bl6 mice. 20 minutes before glucose injection, mice were injected (i.v.) with SPA (8 μg/kg) (square dots) or saline (round dots). Glycemia was measured at time 0 (before injection) and 10, 20, 30, 60, 90, and 120 minutes after glucose injection, from blood samples collected from the caudal vein tail ( for saline and for SPA). (b) The areas under curve (AUC) were calculated using GraphPad Prism from the mean of individual AUC obtained for each mouse. (c) Glycemia was measured from blood samples collected before i.v. injection of SPA () or saline () () and 20 minutes after i.p. glucose injection (). (d) Plasma insulin concentration was measured from the same blood samples as above (, ).

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