Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells

Figure 1

Murine GMC proteome altered by acute (2 h) exposure to HG culture conditions. GMC cells were grown to 80% confluence and were serum-starved (0.5% FBS) overnight, and were treated for 2 h with 25 mM glucose (HG) or 5 mM glucose + 20 mM mannitol () as an isoosmotic control. Cells were lysed using 2DE buffer and 75 μg protein used for 2DE analysis. Proteins whose expression is altered by 2 h HG are annotated on the gel with identifications provided in Table 1. Data are representative of five individual gels for HG and for conditions.