Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 β-Cells Treated with Palmitate and Oleate

Figure 2

Glucose-stimulated insulin secretion in NEFAs-treated MIN6 β-cells. MIN6 β-cells were treated during twenty-four hours with palmitate or oleate (0.5 mM) and then GSIS was evaluated with KRH buffer with or without glucose (20 mM) during one hour. Graph bars represent the insulin secretion at low (white bars) or high glucose (black bars). Graph bars represent mean ± standard errors of measurements of three independent experiments in triplicate. The numbers above bars represent the Stimulation Index (ratio of insulin secreted in cells with and without glucose). The statistical analysis was made comparing treated versus control at basal and stimulated condition. The symbol denotes relative to vehicle condition in one-way ANOVA.