Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 β-Cells Treated with Palmitate and Oleate

Figure 5

Intracellular 5HT content in NEFAs-treated MIN6 β-cells. MIN6 β-cells were supplemented with or without the 5HT precursor 5HTP (50 μM) and treated with palmitate (0.5 mM), oleate (0.5 mM), or the Mao inhibitor Pargyline (20 μM) during twenty-four hours. Then, cells were collected and homogenized and the protein was separated by PCA precipitation. The 5HT concentration was quantified by HPLC in supernatant and adjusted to total protein content (see Section 2). Graph bars represent mean ± standard errors of measurements of three independent experiments in duplicate. The symbol denotes and denotes in one-way ANOVA.