Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 β-Cells Treated with Palmitate and Oleate

Table 2

mRNA expression of serotonin- and dopamine-related genes in islets from db/db mice. Total RNA was extracted from a pool of 335 and 312 islets from WT () and db/db () male mice, respectively. Relative mRNA levels of genes related to the monoamines (MAs) biosynthesis, degradation, and transport were evaluated by RT-qPCR using custom RT-Profiler. The mRNA levels are expressed as fold-change in of the specific gene in islets from db/db relative to WT mice (see Section 2).

Gene	Name	Islets Cts in		Fold change	Related pathway
		WT	db/db
Tph1	Tryptophan hydroxylase 1	33.29	35	0.38	Serotonin biosynthetic pathway
Tph2	Tryptophan hydroxylase 2	31.04	30.27	2.10	Serotonin biosynthetic pathway
Ddc	Dopa decarboxylase	22.7	21.97	2.04	MAs biosynthetic pathway
Aanat	Arylalkylamine N-acetyltransferase	31.37	30.05	3.07	Melatonin biosynthetic pathway
Asmt	Acetylserotonin O-methyltransferase	>35	>35	Not expressed	Melatonin biosynthetic pathway
Th	Tyrosine hydroxylase	28.11	29.13	0.61	Adrenergic biosynthetic pathway
Dbh	Dopamine beta hydroxylase	33.94	35.00	0.59	Adrenergic biosynthetic pathway
Maoa	Monoamine oxidase A	28.33	28.94	0.81	MAs degradative enzymes
Maob	Monoamine oxidase B	23.43	25.17	0.37	MAs degradative enzymes
Comt	Catechol-O-methyltransferase	24.4	24.29	1.30	MAs degradative enzymes
Slc6a3	Solute carrier family 6, member 3	>35	>35	Not expressed	Dopamine transporter
Slc6a4	Solute carrier family 6, member 4	33.17	35.00	0.35	Serotonin transporter
Slc18a1	Solute carrier family 18, member 1; Vmat1	27.29	26.57	2.03	MAs transporters
Slc18a2	Solute carrier family 18, member 2; Vmat2	30.30	29.05	2.93	MAs transporters