Research Article

Involvement of Histone Lysine Methylation in p21 Gene Expression in Rat Kidney In Vivo and Rat Mesangial Cells In Vitro under Diabetic Conditions

Figure 2

H3K9me2/3 levels at p21 gene promoter in the glomeruli of type 1 diabetic rats. Bar graphs showing H3K9me2 and H3K9me3 levels at p21 and CypA promoters in glomeruli of control and STZ groups. ChIP assays were performed with H3K9me2 and H3K9me3 antibodies, immunoprecipitated DNA and input DNA were subjected to RT-qPCR with primers for the respective promoter, data were analyzed by the method, and results normalized to input DNA were expressed as fold over the control group (mean ± SEM; compared to control, /group).