Involvement of Histone Lysine Methylation in p21 Gene Expression in Rat Kidney In Vivo and Rat Mesangial Cells In Vitro under Diabetic Conditions

Figure 5

TGF-β1-specific antibody reversed HG-induced H3K4me1/2/3 levels at p21 gene promoter in RMCs. Bar graphs showing H3K4me1, H3K4me2, and H3K4me3 levels at p21 and CypA gene promoters in RMCs. Serum-depleted RMCs were treated as described in Materials and Methods. ChIP assays were performed as described in Materials and Methods. Results were expressed as fold over NG (mean ± SEM; compared to NG; compared to HG, ).