Treatment with Tacrolimus and Sirolimus Reveals No Additional Adverse Effects on Human Islets In Vitro Compared to Each Drug Alone but They Are Reduced by Adding Glucocorticoids

Figure 2

The effect of immunosuppressant drugs on gene expression of IL-1beta and IL-8 in human islets. Human islets cultured without or with tacrolimus (TAC 30 µg/L), sirolimus (SRL 30 µg/L), or a combination thereof for 24 hours were tested for gene expression of (a) IL-1beta and (b) IL-8 by qPCR in relation to the control gene -actin. Data are presented as mean ± SEM of four separate experiments from four different donors. , , and < 0.0001 versus control.

(a)  

(b)