Treatment with Tacrolimus and Sirolimus Reveals No Additional Adverse Effects on Human Islets In Vitro Compared to Each Drug Alone but They Are Reduced by Adding Glucocorticoids

Figure 4

The effect of methylprednisolone on the inflammatory potential in human islets. Human islets cultured with tacrolimus (TAC 30 µg/L), sirolimus (SRL 30 µg/L), with or without methylprednisolone (MP 1000 ng/L), for 24 hours were tested for (a) gene expression of IL-1beta and IL-8 and (b) protein release of IL-8 and IL-6. The fold change represents the expression of target genes in isolated islets relative to the -actin mRNA level. Data is presented as mean ± SEM of four separate experiments from four different donors. , , and versus control.

(a)  

(b)