Calpain-Calcineurin-Nuclear Factor Signaling and the Development of Atrial Fibrillation in Patients with Valvular Heart Disease and Diabetes

Table 1

Primer sequences were used in qPCR experiments.

Gene	Product size (bp)	Primer sequence
Calpain 2	165	F: 5′-CTGGGGCTGAAGGAGTTCTAC
		R: 5′-GATGACTTGGTGGAGTTGACAG
CnA isoform	112	F: 5′-CAGGAACATTTCACTCACAACACAG
		R: 5′-CGTGGGCTCGGAGTATAGATAACA
CnA isoform	119	F: 5′-TGGATGTCTTCACGTGGTCTTTAC
		R: 5′-ATCAAACTGGTCTTCACCTTCAGTC
NFAT-c3	137	F: 5′-CATCGAGCCCATTATGAAACTGAA
		R: 5′-CGATCATCTGCTGTCCCAATAAAC
NFAT-c4	97	F: 5′-AGCCTGACACACCGTAGGTACTGA
		R: 5′-AGTGCAAATGCCCGGAATG
-actin	366	F: 5′-ACACTGTGCCCATCTACGAGGGG
		R: 5′-ATGAGTGAGTTGAAGGTAGTTTCGTGGAT

CnA, subunit A of calcineurin; F, forward primer; NFAT, nuclear factor of activated T cells; R, reverse primer.