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Journal of Diabetes Research
Volume 2016 (2016), Article ID 4846819, 10 pages
http://dx.doi.org/10.1155/2016/4846819
Research Article

Exogenous Angiotensin I Metabolism in Aorta Isolated from Streptozotocin Treated Diabetic Rats

1Department of Pharmacology, Jagiellonian University Medical College, Krakow, Poland
2Center for Medical Genomics OMICRON, Jagiellonian University Medical College, Krakow, Poland
3Department of Pharmacology, Medical Faculty, University of Rzeszów, Rzeszów, Poland

Received 1 July 2016; Accepted 31 August 2016

Academic Editor: Raffaele Marfella

Copyright © 2016 P. P. Wołkow et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. Products of angiotensin (ANG) I metabolism may predispose to vascular complications of diabetes mellitus. Methods. Diabetes was induced with streptozotocin (75 mg/kg i.p.). Rat aorta fragments, isolated 4 weeks later, were pretreated with perindoprilat (3 μM), thiorphan (3 μM), or vehicle and incubated for 15 minutes with ANG I (1 μM). Products of ANG I metabolism through classical (ANG II, ANG III, and ANG IV) and alternative (ANG (1–9), ANG (1–7), and ANG (1–5)) pathways were measured in the buffer, using liquid chromatography-mass spectrometry. Results. Incubation with ANG I resulted in higher concentration of ANG II (P = 0.02, vehicle pretreatment) and lower of ANG (1–9) (, perindoprilat pretreatment) in diabetes. Preference for the classical pathway is suggested by higher ANG III/ANG (1–7) ratios in vehicle (), perindoprilat (), and thiorphan pretreated () diabetic rat. Within the classical pathway, ratios of ANG IV/ANG II () and of ANG IV/ANG III (), but not of ANG III/ANG II are lower in diabetes. Conclusions. Diabetes in rats led to preference toward deleterious (ANG II, ANG III) over protective (ANG IV, ANG (1–9), and ANG (1–7)) ANG I metabolites.