Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro

Figure 10

Podocyte motility by wound-healing assay and transwell migration assay. The wound-healing assay (a) and the modified transwell migration assay (b) were performed. Podocytes showed little migration when incubated in 5.5 mM glucose (column 1). Stimulation with 30 mM glucose-induced increased migration of podocytes (column 2), which was partially decreased by transfection with Fyn siRNA for 48 hours (column 4) or pretreatment with 10 μM Y27632 for 30 minutes (column 6). Control podocytes were treated with Cont-siRNA (column 5) or 5.5 mM glucose plus 24.5 mM mannitol (column 3). Magnification: 200x.

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