Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro

Figure 5

Western blot analysis of alterations to Fyn expression and Fyn activation in podocytes. Levels of activated Fyn exhibited a remarkable upregulation after 48-hour incubation with 30 mM glucose (lane 2) compared with cells incubated with 5.5 mM glucose (lane 1). Fyn siRNA significantly downregulated Fyn expression and activity (lane 4), whereas pretreatment with 10 μM Y27632 for 30 minutes showed little effects on Fyn activation (lane 5). Control podocytes were treated with 5.5 mM glucose plus 24.5 mM mannitol for 48 hours (lane 3) or transfected with Cont-siRNA (lane 5). Values denote the mean ± SD; versus 5.5 mM glucose and versus 30 mM glucose.