Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro

Figure 8

Western blotting analysis of the phosphorylation levels of paxillin in podocytes. The phosphorylation of paxillin (PY31) was significantly increased after a 48-hour incubation with 30 mM glucose (lane 2) compared with cells incubated with 5.5 mM glucose (lane 1), and no alterations in total paxillin expression were noted. Transfection with Fyn siRNA for 24 hours (lane 4) or pretreatment with 10 μM Y27632 for 30 minutes (lane 6) partially decreased high glucose-induced paxillin phosphorylation. Control podocytes were treated with Cont-siRNA (lane 5) or 5.5 mM glucose plus 24.5 mM mannitol (lane 3). Values denote the mean ± SD; versus 5.5 mM glucose and versus 30 mM glucose.