In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications

Figure 2

Insulin expression in islet-derived cells in culture. The immunostaining images were obtained from intact pancreatic islet tissue (a), islet-derived cells immediately after islet digestion (b), and after 3 days (c) and 6 (d) days in culture, at 8 days after P1 (e) and at 4 days after P2. Colors show insulin (red) and DAPI (blue) staining. Scale bars = 100 μm (a–d) and 50 μm (e, f).

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